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Part:BBa_K136040

Designed by: alexandra bouaziz   Group: iGEM08_Paris   (2008-10-25)


pFlgA-RFP

Promoter of flgA gene from E. coli flagella

flgA is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).

Its promoter is consitutively repressed.

It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).

flgA promotor is here followed by RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3137
    Illegal XbaI site found at 3152
    Illegal SpeI site found at 212
    Illegal PstI site found at 1095
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3137
    Illegal SpeI site found at 212
    Illegal PstI site found at 1095
    Illegal NotI site found at 1088
    Illegal NotI site found at 3143
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3137
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3137
    Illegal XbaI site found at 3152
    Illegal SpeI site found at 212
    Illegal PstI site found at 1095
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3137
    Illegal XbaI site found at 3152
    Illegal SpeI site found at 212
    Illegal PstI site found at 1095
    Illegal AgeI site found at 791
    Illegal AgeI site found at 903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2176


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